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KMID : 1098420030110040298
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Korean Journal of Medicinal Crop Science
2003 Volume.11 No. 4 p.298 ~ p.305
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Methanol Extract of Polygalae Radix Protects Excitotoxicity in Cultured Neuronal Cells
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Ban, Ju Yeon
Lee, Hyun Joo/Lee, Soo Bae/Lee, Young Jong/Seong, Nak Sul/Song, Kyung Sik/Bae, Ki Whan
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Abstract
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Polygalae Radix (PR) from Polygala tenuifolia (Polygalaceae) is traditionally used in China and Korea, since this herb has a sedative, antiinflammatory, and antibacterial agent. To extend pharmacological actions of PR in the CNS on the basis of its CNS inhibitory effect, the present study examined whether PR has the neuroprotective action against kainic acid (KA)-induced cell death in primarily cultured rat cerebellar granule neurons. PR, over a concentration range of 0.05 to 5 ㎍/㎖, inhibited KA (500 μM)-induced neuronal cell death, which was measured by a trypan blue exclusion test and a 3-[4,5-dimethylthiazol-2-yl]2,5-diphenyl-tetrazolium bromide (MTT) assay. PR (0.5 ㎍/㎖) inhibited glutamate release into medium induced by KA (500 μM), which was measured by HPLC. Pretreatment of PR (0.5 ㎍/㎖) inhibited KA (500 μM)-induced elevation of cytosolic calcium concentration ([Ca^(²+)]_(c)), which was measured by a fluorescent dye, Fura 2-AM, and generation of reactive oxygen species (ROS). These results suggest that PR prevents KA-induced neuronal cell damage in vitro.
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